鉴定抑制人乳头瘤病毒11型E2蛋白的DNA结合、反式激活和DNA复制功能的肽段。
Identification of peptides that inhibit the DNA binding, trans-activator, and DNA replication functions of the human papillomavirus type 11 E2 protein.
作者信息
Deng Su-Jun, Pearce Kenneth H, Dixon Eric P, Hartley Kelly A, Stanley Thomas B, Lobe David C, Garvey Edward P, Kost Thomas A, Petty Regina L, Rocque Warren J, Alexander Kenneth A, Underwood Mark R
机构信息
Departments of Gene Expression and Protein Biochemistry, GlaxoSmithKline Research and Development, Research Triangle Park, North Carolina 27709, USA.
出版信息
J Virol. 2004 Mar;78(5):2637-41. doi: 10.1128/jvi.78.5.2637-2641.2003.
Peptide antagonists of the human papillomavirus type 11 (HPV-11) E2-DNA association were identified using a filamentous bacteriophage random peptide library. Synthetic peptides antagonized the E2-DNA interaction, effectively blocked E2-mediated transcriptional activation of a reporter gene in cell culture, and inhibited E1-E2-mediated HPV-11 DNA replication in vitro. These peptides may prove to be useful tools for characterizing E2 function and for exploring the effectiveness of E2-inhibitor-based treatments for HPV-associated diseases.
利用丝状噬菌体随机肽库鉴定出了人乳头瘤病毒11型（HPV - 11）E2 - DNA结合的肽拮抗剂。合成肽拮抗E2 - DNA相互作用，有效阻断细胞培养中报告基因的E2介导的转录激活，并在体外抑制E1 - E2介导的HPV - 11 DNA复制。这些肽可能被证明是用于表征E2功能以及探索基于E2抑制剂的治疗方法对HPV相关疾病有效性的有用工具。
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