Wang Yan, Ma Wen-li, Mao Xiang-ming, Wu Qing-hua, Li Ling, Wang Hong-min, Xiao Wei-wei, Zheng Wen-ling
Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2004 Feb;24(2):180-3.
To study the preparation of oligonucleotide microarray for detecting vaccinia virus.
Oligonucleotide probes were designed and synthesized according to the specific genes of vaccinia virus. Sample DNA of the virus and the negative control sample were obtained and labeled by restriction display technique, followed by hybridization to the oligonucleotide microarray and scanned by Agilent scanner.
Strong hybridization signals were detected from the viral DNA hybridized with the microarray, but were absent in the negative sample when positive probes were not used.
Distinct differences in the hybridization signals between the virus sample and negative sample and between the samples obtained in different phase of infection demonstrate high specificity and sensitivity of the microarray for vaccinia virus detection.
研究用于检测痘苗病毒的寡核苷酸微阵列的制备方法。
根据痘苗病毒的特定基因设计并合成寡核苷酸探针。获取病毒的样本DNA和阴性对照样本，采用限制性显示技术进行标记，然后与寡核苷酸微阵列杂交，并用安捷伦扫描仪进行扫描。
与微阵列杂交的病毒DNA检测到强杂交信号，但在未使用阳性探针时，阴性样本中无信号。
病毒样本与阴性样本之间以及感染不同阶段获得的样本之间杂交信号存在明显差异，表明该微阵列用于痘苗病毒检测具有高特异性和高灵敏度。
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