Uekita Takamasa, Kim Yong-Jin, Yamanouchi Keitaro, Tojo Hideaki, Tachi Chikashi
Laboratory of Applied Genetics, Department of Animal Resource Science, School of Agriculture and Life Sciences, The University of Tokyo, Japan.
J Reprod Dev. 2003 Jun;49(3):243-52. doi: 10.1262/jrd.49.243.
Changes in the betaig-h3 gene expression levels in the uterus during different reproductive stages and those in the placenta on different days of gestation in the mouse were examined by Northern blot analysis. The levels of betaig-h3 expression rose steeply from the baseline level at proestrus to the maximum at estrus and then declined rapidly from 1st day of diestrus onward. During pregnancy, high levels of betaig-h3 mRNA were detected in the uterus on Day 4 of gestation, when the trophoblastic invasion of the implanted blastocysts was very active. In the pseudopregnant uterus, the betaig-h3 expression levels exhibited the same patterns as those in the pregnant uterus, although the levels were much lower at all stages than those in the pregnant uterus. In the placenta, the betaig-h3 expression levels gradually increased from Day 7 onward and peaked on Day 18 of gestation. In situ hybridization analysis of the pregnant uterine tissues revealed that the luminal as well as the glandular epithelium expressed betaig-h3 mRNA at high levels. In the placenta, high levels of betaig-h3 transcripts were detected in the decidual cells and the giant trophoblast cells. These findings suggest that betaig-h3 might play a role in regulating the invasiveness of trophoblast cells during implantation and placentation of hemochorial type when extensive trophoblastic invasion of the endometrium takes place.
通过Northern印迹分析检测了小鼠在不同生殖阶段子宫中βig - h3基因表达水平的变化以及妊娠不同天数胎盘组织中βig - h3基因表达水平的变化。βig - h3的表达水平从发情前期的基线水平急剧上升至发情期的最高水平，然后从黄体期第1天开始迅速下降。在妊娠期间，妊娠第4天子宫中检测到高水平的βig - h3 mRNA，此时植入的胚泡滋养层细胞侵入非常活跃。在假孕子宫中，βig - h3的表达水平呈现出与妊娠子宫相同的模式，尽管在所有阶段其水平均远低于妊娠子宫。在胎盘中，βig - h3的表达水平从第7天开始逐渐升高，并在妊娠第18天达到峰值。对妊娠子宫组织进行原位杂交分析显示，腔上皮和腺上皮均高水平表达βig - h3 mRNA。在胎盘中，蜕膜细胞和巨大滋养层细胞中检测到高水平的βig - h3转录本。这些发现表明，βig - h3可能在血绒毛膜型植入和胎盘形成过程中调节滋养层细胞的侵袭性方面发挥作用，此时子宫内膜会发生广泛的滋养层细胞侵入。
