Paukert Martin, Sidi Samuel, Russell Claire, Siba Maria, Wilson Stephen W, Nicolson Teresa, Gründer Stefan
Department of Physiology II and Max-Planck-Institute of Developmental Biology, 72076 Tübingen, Germany.
J Biol Chem. 2004 Apr 30;279(18):18783-91. doi: 10.1074/jbc.M401477200. Epub 2004 Feb 16.
Acid-sensing ion channels (ASICs) are excitatory receptors for extracellular H(+). Proposed functions include synaptic transmission, peripheral perception of pain, and mechanosensation. Despite the physiological importance of these functions, the precise role of ASICs has not yet been established. In order to increase our understanding of the physiological role and basic structure-function relationships of ASICs, we report here the cloning of six new ASICs from the zebrafish (zASICs). zASICs possess the basic functional properties of mammalian ASICs: activation by extracellular H(+), Na(+) selectivity, and block by micromolar concentrations of amiloride. The zasic genes are broadly expressed in the central nervous system, whereas expression in the peripheral nervous system is scarce. This pattern suggests a predominant role for zASICs in neuronal communication. Our results suggest a conserved function for receptors of extracellular H(+) in the central nervous system of vertebrates.
酸敏感离子通道（ASICs）是细胞外H⁺的兴奋性受体。其推测的功能包括突触传递、外周痛觉感知和机械感觉。尽管这些功能在生理学上很重要，但ASICs的确切作用尚未确定。为了增进我们对ASICs生理作用及基本结构 - 功能关系的理解，我们在此报告从斑马鱼中克隆出六种新的ASICs（zASICs）。zASICs具有哺乳动物ASICs的基本功能特性：被细胞外H⁺激活、对Na⁺有选择性以及被微摩尔浓度的氨氯吡咪阻断。zasic基因在中枢神经系统中广泛表达，而在外周神经系统中的表达很少。这种模式表明zASICs在神经元通讯中起主要作用。我们的结果表明细胞外H⁺受体在脊椎动物中枢神经系统中具有保守功能。
