Pollak Arthur, Rokach Ariel, Blumenfeld Anat, Rosen Laura J, Resnik Luba, Dresner Pollak Rivka
Department of Cardiology, Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
Eur Heart J. 2004 Feb;25(3):240-5. doi: 10.1016/j.ehj.2003.10.028.
To investigate the association between sequence variants in the promoter region of the oestrogen receptor-alpha (ER-alpha) gene and the angiographic severity of coronary artery disease (CAD).
We studied 503 subjects undergoing coronary angiography (mean age 63+/-12 years, 72% men, 28% women). Coronary artery disease extent was assessed by the number of: (1) major coronary vessels with >50% narrowing (NMCV); (2) coronary vessels with any narrowing (NCV); (3) narrowed coronary segments (NCS). The number of thymine and adenine dinucleotide repeats [(TA)(n)], 1174 base-pairs upstream exon 1, was determined by PCR. The median number of (TA)(n)(18) was used to categorize subjects into long, short and mixed allele genotypes. Poisson regression was used to analyse the association between genotypes and CAD extent, with age category (age #10877;55 vs >55), sex, risk factors and age at onset of CAD as covariates. In young subjects, (TA)(n)length had a significant effect on NCS (P=0.047) and a borderline significant effect on NCV (P=0.066). Young subjects homozygous for long alleles had higher NCV and NCS compared to those homozygous for short alleles (NCV 3.7+/-2.4 vs 2.4+/-1.8, NCS 4.4+/-2.7 vs 3.1+/-2.3, respectively, P#10877;0.034).
The (TA)(n)length in the ER-alpha gene promoter region is associated with the angiographic severity of CAD in young patients.
研究雌激素受体α（ER-α）基因启动子区域的序列变异与冠状动脉疾病（CAD）血管造影严重程度之间的关联。
我们研究了503例接受冠状动脉造影的受试者（平均年龄63±12岁，男性72%，女性28%）。通过以下数量评估冠状动脉疾病程度：（1）狭窄>50%的主要冠状动脉血管数量（NMCV）；（2）有任何狭窄的冠状动脉血管数量（NCV）；（3）狭窄的冠状动脉节段数量（NCS）。通过聚合酶链反应（PCR）确定位于外显子1上游1174个碱基对处的胸腺嘧啶和腺嘌呤二核苷酸重复序列[（TA）（n）]的数量。以（TA）（n）的中位数（18）将受试者分为长等位基因、短等位基因和混合等位基因基因型。采用泊松回归分析基因型与CAD程度之间的关联，将年龄类别（年龄≤55岁与>55岁）、性别、危险因素和CAD发病年龄作为协变量。在年轻受试者中，（TA）（n）长度对NCS有显著影响（P = 0.047），对NCV有临界显著影响（P = 0.066）。与短等位基因纯合子相比，长等位基因纯合子的年轻受试者NCV和NCS更高（NCV分别为3.7±2.4与2.4±1.8，NCS分别为4.4±2.7与3.1±2.3，P<0.034）。
ER-α基因启动子区域的（TA）（n）长度与年轻患者CAD的血管造影严重程度相关。
