被误认作THP-1细胞的Raji B细胞会刺激DC-SIGN介导的HIV传播。

Raji B cells, misidentified as THP-1 cells, stimulate DC-SIGN-mediated HIV transmission.

作者信息

Wu Li, Martin Thomas D, Carrington Mary, KewalRamani Vineet N

机构信息

Model Development Section, HIV Drug Resistance Program, National Cancer Institute at Frederick, National Institutes of Health, Frederick, MD 21702, USA.

出版信息

Virology. 2004 Jan 5;318(1):17-23. doi: 10.1016/j.virol.2003.09.028.

DOI:10.1016/j.virol.2003.09.028

PMID:14972530

Abstract

A number of studies examining interactions of dendritic cell (DC)-specific ICAM-3 grabbing nonintegrin (DC-SIGN) with viral pathogens have relied on monocytic transfectants as models for primary DCs. Here we show that the presumed "THP-1" monocytic cells used in these studies are instead Raji B cells. Moreover, we demonstrate that true THP-1 cells do not support DC-SIGN-mediated HIV-1 transmission, whereas human B cell lines efficiently enhance this process. These data indicate that there are features common to B cells and DCs that facilitate transmission of HIV-1 and provide new insights toward the mechanism of DC-SIGN-mediated HIV-1 transmission.

摘要

许多研究通过树突状细胞（DC）特异性细胞间黏附分子3抓取非整合素（DC-SIGN）与病毒病原体的相互作用，将单核细胞转染体作为原代DC的模型。在此我们表明，这些研究中使用的所谓“THP-1”单核细胞实际上是Raji B细胞。此外，我们证明真正的THP-1细胞不支持DC-SIGN介导的HIV-1传播，而人类B细胞系可有效增强这一过程。这些数据表明，B细胞和DC具有共同特征，有助于HIV-1传播，并为DC-SIGN介导的HIV-1传播机制提供了新见解。