Clarkson Richard W E, Watson Christine J
Department of Pathology, University of Cambridge, Cambridge, United Kingdom.
J Mammary Gland Biol Neoplasia. 2003 Jul;8(3):309-19. doi: 10.1023/b:jomg.0000010031.53310.92.
Mammary epithelial cells (MEC) undergo a series of developmental decisions during a pregnancy cycle. The switches from proliferation to differentiation to secretion and then to cell death are precisely controlled. In order to identify critical changes associated with the transition from a secretory phenotype during lactation to dedifferentiation and cell death, we have undertaken a microarray analysis of mouse mammary gland development. We have focused on the involution switch and on the transcription profiles of genes that are targets of transcription factors known to influence involution and apoptosis. Our results show that both Stat3 and NF-kB target genes are induced by the involution switch while Stat5 target genes are distinct from Stat3 induced genes. Furthermore, a substantial number of genes that were specifically upregulated at the start of involution are regulators of inflammation and the acute phase response. These results provide a novel insight into the involution process and demonstrate the value of microarray analysis in defining molecular events associated with critical developmental transitions in mammary gland.
乳腺上皮细胞（MEC）在妊娠周期中经历一系列发育过程。从增殖到分化再到分泌，然后到细胞死亡的转变受到精确控制。为了确定与从泌乳期的分泌表型转变为去分化和细胞死亡相关的关键变化，我们对小鼠乳腺发育进行了微阵列分析。我们重点关注退化转变以及已知影响退化和细胞凋亡的转录因子靶基因的转录谱。我们的结果表明，Stat3和NF-kB靶基因均由退化转变诱导，而Stat5靶基因与Stat3诱导的基因不同。此外，在退化开始时特异性上调的大量基因是炎症和急性期反应的调节因子。这些结果为退化过程提供了新的见解，并证明了微阵列分析在定义与乳腺关键发育转变相关的分子事件中的价值。
