利用重复聚合酶链反应（rep-PCR）技术测定密执安棒杆菌密歇根亚种流体变种和非流体变种之间的遗传差异。
Determination of genetic differences between fluid and nonfluid variants of Clavibacter michiganensis subsp. sepedonicus using rep-PCR technique.
作者信息
Fousek J, Mráz I
机构信息
Department of Plant Virology, Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, 370 05 Ceské Budĕjovice, Czechia.
出版信息
Folia Microbiol (Praha). 2003;48(5):682-6. doi: 10.1007/BF02993479.
PMID:14976729
Abstract
Testing of 23 isolates of Clavibacter michiganensis subsp. sepedonicus for analysis by rep-PCR (using BOX, ERIC, REP primer sets) was used for the purpose of localization of genetic markers for fluid and/or nonfluid strains. None of the primer sets was successful in detecting genetic differences between the isolates and no polymorphism was generated.
摘要
对23株密执安棒杆菌亚种马铃薯环腐病菌进行rep-PCR分析（使用BOX、ERIC、REP引物组），目的是定位液体和/或非液体菌株的遗传标记。没有一组引物能够成功检测出这些分离株之间的遗传差异，也未产生多态性。
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