Gondet L, Weber T, Maillot-Vernier P, Benveniste P, Bach T J
Centre National de la Recherche Scientifique, Institut de Botanique, Strasbourg, France.
Biochem Biophys Res Commun. 1992 Jul 31;186(2):888-93. doi: 10.1016/0006-291x(92)90829-a.
In a tobacco mutant callus, containing up to tenfold more sterols than the wild-type genotype, HMG-CoA reductase activity is increased by a factor of approximately three, as is the case in mutant seedlings and plants. The rate of HMG-CoA synthesis from acetyl-CoA by the coupled enzyme system acetoacetyl-CoA thiolase/HMG-CoA synthase, as well as its conversion to acetyl-CoA plus acetoacetate by action of HMG-CoA lyase are not affected. These results confirm the key-regulating role of HMG-CoA reductase in sterol biosynthesis, which seems not to be confined only to the animal kingdom, but can also be extended to plants.
在一种烟草突变体愈伤组织中，其甾醇含量比野生型基因型多高达十倍，3-羟基-3-甲基戊二酰辅酶A还原酶（HMG-CoA还原酶）的活性增加了约三倍，突变体幼苗和植株的情况也是如此。由乙酰辅酶A通过乙酰乙酰辅酶A硫解酶/HMG-CoA合酶偶联酶系统合成HMG-CoA的速率，以及通过HMG-CoA裂解酶的作用将其转化为乙酰辅酶A和乙酰乙酸的速率均未受影响。这些结果证实了HMG-CoA还原酶在甾醇生物合成中的关键调节作用，这似乎不仅局限于动物界，也可扩展到植物界。
