Dong Xiao-xian, Liu Jin-bao, Dong Yan-xiang
Department of Pathophysiology, Guangzhou Medical College, Guangzhou 510182.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2004 Jan;24(1):51-4.
To investigate the effect of gastrodia in extracorporeal oriented inducing the differentiation of mesenchymal stem cells into neuron-like cells.
Mesenchymal stem cells were separated from bone marrow of rats by wall sticking method, amplifying cultured in vitro, and differentiated into neuron-like cells by oriented induction with gastrodia. The morphology of cells was observed under light microscopy, neuro-specific enolase (NSE), nestin and glial fibrillary acidic protein (GFAP) were detected by immunocytochemistry.
Rats mesenchymal stem cells could be separated and amplified in vitro. After being induced by gastrodia for 2 hrs, most of the cells would be differentiated into meuron-like cells, revealing cytodendrite. By immunochemical staining, cells showed positive of NSE, nestin, and negative of GFAP.
Rats' mesenchymal stem cells could be induced to differentiate into neuron-like cells.
探讨天麻在体外定向诱导间充质干细胞分化为神经元样细胞中的作用。
采用贴壁法从大鼠骨髓中分离间充质干细胞，体外扩增培养，并用天麻进行定向诱导使其分化为神经元样细胞。在光学显微镜下观察细胞形态，采用免疫细胞化学法检测神经特异性烯醇化酶（NSE）、巢蛋白和胶质纤维酸性蛋白（GFAP）。
大鼠间充质干细胞可在体外分离并扩增。经天麻诱导2小时后，大部分细胞可分化为神经元样细胞，呈现出细胞树突。免疫化学染色显示，细胞NSE、巢蛋白呈阳性，GFAP呈阴性。
大鼠间充质干细胞可被诱导分化为神经元样细胞。
