以阳离子花菁为荧光探针测定核酸的荧光增强法

Fluorescence enhancement method for the determination of nucleic acids using cationic cyanine as a fluorescence probe.

作者信息

Zhu Chang-Qing, Zhuo Shu-Juan, Zheng Hong, Chen Jin-Long, Li Dong-Hui, Li Shun-Hua, Xu Jin-Gou

机构信息

The Key Laboratory of Analytical Science of MOE, Department of Chemistry, Xiamen University, Xiamen 361005, China.

出版信息

Analyst. 2004 Mar;129(3):254-8. doi: 10.1039/b313463f. Epub 2004 Jan 23.

DOI:10.1039/b313463f

PMID:14978529

Abstract

A fluorescence enhancement method with a cationic cyanine as a probe was developed for the determination of nucleic acids. Under the experimental conditions, the fluorescence enhancement of cyanine (lambda(ex)/lambda(em)= 524/591.5 nm) was observed in the presence of DNA. The calibration graphs were linear over the range of 0.01-15 microg mL(-1) for both calf thymus DNA (CT DNA) and fish sperm DNA (FS DNA). The limits of detection were 0.005 and 0.007 microg mL(-1) for CT DNA and FS DNA, respectively. The method was applied to the determination of DNA in synthetic and real samples and satisfactory results were obtained. A possible fluorescence enhancement mechanism was also studied.

摘要

开发了一种以阳离子花菁为探针的荧光增强法用于核酸的测定。在实验条件下，在DNA存在时观察到花菁（激发波长/发射波长 = 524/591.5 nm）的荧光增强。小牛胸腺DNA（CT DNA）和鱼精DNA（FS DNA）的校准曲线在0.01 - 15 μg mL⁻¹范围内呈线性。CT DNA和FS DNA的检测限分别为0.005和0.007 μg mL⁻¹。该方法应用于合成样品和实际样品中DNA的测定，获得了满意的结果。还研究了一种可能的荧光增强机制。