新型油包水微乳液在微乳液电动色谱中的应用。

The use of novel water-in-oil microemulsions in microemulsion electrokinetic chromatography.

作者信息

Altria Kevin D, Broderick Margo F, Donegan Sheila, Power Joe

机构信息

Pharmaceutical Development GlaxoSmithKline R&D, Harlow, Essex, UK.

出版信息

Electrophoresis. 2004 Feb;25(4-5):645-52. doi: 10.1002/elps.200305789.

DOI:10.1002/elps.200305789

PMID:14981692

Abstract

We describe the novel use of water-in-oil (W/O) microemulsions to achieve unique separations in microemulsion electrokinetic chromatography (MEEKC). The choice and concentration of the buffer type, surfactant and co-surfactant were all examined and optimized. Separations of a range of neutral and acidic analytes was shown to be markedly different to that obtained by (oil-in-water) O/W MEEKC. Neutral solutes are separated by virtue of their solubility (log P) values in O/W MEEKC with the more water-insoluble solutes migrating last. This separation process does not occur in W/O, as neutral solutes are not separated in order of log P.

摘要

我们描述了油包水（W/O）微乳液在微乳液电动色谱（MEEKC）中实现独特分离的新用途。对缓冲液类型、表面活性剂和助表面活性剂的选择及浓度进行了考察和优化。一系列中性和酸性分析物的分离结果显示与水包油（O/W）型MEEKC明显不同。在O/W型MEEKC中，中性溶质凭借其在其中的溶解度（log P）值进行分离，疏水性更强的溶质最后迁移。而在W/O型微乳液电动色谱中不会发生这种分离过程，因为中性溶质不是按照log P的顺序进行分离的。