单-L-天冬酰胺基二氢卟吩e6（NPe6）对肠道肿瘤的光动力治疗：一项基础研究。
Photodynamic therapy of intestinal tumors with mono-L-aspartyl chlorin e6 (NPe6): a basic study.
作者信息
Kikuchi Tatsuya, Asakura Tohru, Aihara Hiroyuki, Shiraki Manabu, Takagi Sho, Kinouchi Yoshitaka, Aizawa Katsuo, Shimosegawa Tooru
机构信息
Division of Gastroenterology, Tohoku University Graduate School of Medicine, 1-1 Seiryo, Aoba, Sendai 980-8574, Japan.
出版信息
Anticancer Res. 2003 Nov-Dec;23(6C):4897-900.
BACKGROUND
To clarify the usefulness of the second-generation photosensitizer, mono-L-aspartyl chlorin e6 (NPe6), we examined the possibility of photodynamic diagnosis and photodynamic therapy for intestinal tumors in a mouse model.
MATERIALS AND METHODS
NPe6 was intravenously administered to the tumor-bearing mice through the tail vein. The intestinal tumor sites were irradiated with a 664-nm diode laser at constant intervals after the administration of photosensitizers. The tumors were excised and fluorescence was observed in frozen sections by microscope.
RESULTS
We observed the fluorescent image and calculated that the mean fluorescence intensity was significantly higher in the tumors than the normal mucosa during 6 hours (p < 0.05). The fluorescence of NPe6 was chiefly accumulated in the intestinal tumors as red fluorescence on the fluorescent microphotographic image.
CONCLUSION
We conclude that NPe6 may be a valuable photosensitizer for the photodynamic diagnosis and photodynamic therapy of intestinal tumors.
背景
为阐明第二代光敏剂单-L-天冬酰胺基二氢卟吩e6（NPe6）的效用，我们在小鼠模型中研究了对肠道肿瘤进行光动力诊断和光动力治疗的可能性。
材料与方法
通过尾静脉向荷瘤小鼠静脉注射NPe6。在给予光敏剂后，以固定间隔用664纳米二极管激光照射肠道肿瘤部位。切除肿瘤，通过显微镜在冰冻切片中观察荧光。
结果
我们观察到荧光图像，并计算出在6小时内肿瘤中的平均荧光强度显著高于正常黏膜（p < 0.05）。在荧光显微图像上，NPe6的荧光主要以红色荧光形式积聚在肠道肿瘤中。
结论
我们得出结论，NPe6可能是用于肠道肿瘤光动力诊断和光动力治疗的一种有价值的光敏剂。
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