在27兆赫兹石英晶体微天平上对酶促葡聚糖水解进行直接监测。
Direct monitoring of enzymatic glucan hydrolysis on a 27-MHz quartz-crystal microbalance.
作者信息
Nishino Hidekazu, Nihira Takanori, Mori Toshiaki, Okahata Yoshio
机构信息
Department of Biomolecular Engineering, Tokyo Institute of Technology and CREST, JST, Nagatsuda, Midori-ku, Yokohama 226-8501, Japan.
出版信息
J Am Chem Soc. 2004 Mar 3;126(8):2264-5. doi: 10.1021/ja0361805.
PMID:14982404
Abstract
By using a highly sensitive 27-MHz glucan-immobilized quartz-crystal microbalance, we could follow kinetically all processes (enzyme binding and release, kon and koff, and intramolecular hydrolysis rates, kcat) of glucan hydrolysis by glucoamylase by detecting directly the formation and decay of the enzyme-substrate complex as mass changes.
摘要
通过使用高灵敏度的27兆赫葡聚糖固定化石英晶体微天平，我们能够通过直接检测酶 - 底物复合物的形成和衰变（以质量变化表示），动态跟踪葡糖淀粉酶水解葡聚糖的所有过程（酶的结合与释放、结合速率常数kon和解离速率常数koff以及分子内水解速率kcat）。
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