澳大利亚潜在致病性阿米巴鞭毛虫意大利耐格里虫的检测及其意义

Detection and significance of the potentially pathogenic amoeboflagellate Naegleria italica in Australia.

作者信息

Robinson Bret S, Monis Paul T, Henderson Mandy, Gelonese Stella, Ferrante Antonio

机构信息

Australian Water Quality Centre, SA Water Corporation, Private Mail Bag 3, Salisbury, SA 5108, Australia.

出版信息

Parasitol Int. 2004 Mar;53(1):23-7. doi: 10.1016/j.parint.2003.10.002.

DOI:10.1016/j.parint.2003.10.002

PMID:14984832

Abstract

Thermophilic amoeboflagellates in the genus Naegleria include both virulent and benign species. One of the less studied species, N. italica, has not been detected in the environment since the first reports from Italy in the 1980s; its virulence is known only from infection of laboratory mice. Two recent strains from recreational water in Western Australia (AWQC NG960, NG961) were tentatively identified as N. italica from the characteristic mobilities of seven isozymes. Sequences of the 5.8S rRNA gene and its flanking ITS aligned with a 380+bp length of the published sequence for N. italica with 98% identity. Differences from the type strain were confined to ITS2. Shorter alignments (<320 bp) were observed with other Naegleria species, corresponding to conserved regions of the 5.8S gene and ITS. Unlike the European type strain of N. italica, the Australian isolates failed to infect laboratory mice intranasally, confirming that infectivity of this species is variable and often lower than in N. fowleri.

摘要

嗜热变形鞭毛虫属的纳格里亚属包括致病和良性物种。其中一个研究较少的物种，意大利纳格里亚，自20世纪80年代意大利首次报道以来，在环境中尚未被检测到；其致病性仅在实验室小鼠感染中已知。最近从西澳大利亚休闲水中分离出的两株菌株（AWQC NG960、NG961），根据七种同工酶的特征迁移率，初步鉴定为意大利纳格里亚。5.8S rRNA基因及其侧翼ITS的序列与已发表的意大利纳格里亚序列的380多个碱基对长度比对，同一性为98%。与模式菌株的差异仅限于ITS2。与其他纳格里亚物种的比对较短（<320碱基对），对应于5.8S基因和ITS的保守区域。与欧洲的意大利纳格里亚模式菌株不同，澳大利亚分离株经鼻内接种未能感染实验室小鼠，证实该物种的感染性具有变异性，且通常低于福氏纳格里亚。