Epand Richard M, Vuong Phan, Yip Christopher M, Maekawa Shohei, Epand Raquel F
Department of Biochemistry, McMaster University, Hamilton L8N 3Z5, ON, Canada.
Biochem J. 2004 May 1;379(Pt 3):527-32. doi: 10.1042/BJ20040204.
A myristoylated peptide corresponding to the N-terminus of NAP-22 (neuronal axonal myristoylated membrane protein of 22 kDa) causes the quenching of the fluorescence of BODIPY-TMR-labelled PtdIns(4,5) P2 in bilayers of 1-palmitoyl-2-oleoyl phosphatidylcholine containing 40 mol% cholesterol and 0.1 mol% BODIPY-PtdIns(4,5)2. Both fluorescence spectroscopy and total internal reflectance fluorescence microscopy revealed the cholesterol-dependent nature of PtdIns(4,5) P2-enriched membrane-domain formation.
一种与NAP-22(22 kDa神经元轴突肉豆蔻酰化膜蛋白)N端对应的肉豆蔻酰化肽,可使含40 mol%胆固醇和0.1 mol%BODIPY-PtdIns(4,5)2的1-棕榈酰-2-油酰磷脂酰胆碱双层膜中BODIPY-TMR标记的PtdIns(4,5)P2荧光淬灭。荧光光谱法和全内反射荧光显微镜均揭示了富含PtdIns(4,5)P2的膜结构域形成的胆固醇依赖性本质。