Zhao Zhong-sheng, Ru Guo-qing, Ma Jie
Department of Pathology, Zhejiang Provincial Hospital, Hangzhou 310014, China.
Zhonghua Bing Li Xue Za Zhi. 2004 Feb;33(1):16-20.
To investigate mRNA expression of integrin beta3 in gastric carcinoma, its correlation with microvascular density (MVD), growth-pattern, invasion, metastasis and prognosis.
In situ hybridization and immunohistochemistry techniques were used to study the expression of integrin beta3 mRNA and CD34 protein in 105 gastric carcinoma specimens.
In situ hybridization revealed a 30% (6/20) positive rate of integrin beta3 mRNA in non-tumor gastric mucosa, which was significantly lower than that of the gastric cancer group (61.0%, 64/105, chi(2) = 8.85, P = 0.037); In infiltrating type cases (70.2%, 40/57), stage III - IV (72.1%, 44/61), lymphatic metastasis (68.6%, 48/70) and distant metastasis (85.7%, 36/42), the positive expression rates were significantly higher than those of the expanding type (chi(2) = 14.97, P = 0.002), stage I - II (chi(2) = 15.21, P = 0.015), non-lymphatic metastasis (chi(2) = 17.89, P = 0.025) and non-distant metastasis (chi(2) = 20.22, P = 0.005; chi(2) = 21.35, P = 0.035); its mean MVD was also significantly higher than that of the expanding type (t = 10.105, P = 0.001), stage I approximately II (t = 5.961, P = 0.001), non-lymphatic metastasis (t = 3.819, P = 0.01)and non-distant metastasis (t = 10.578, P = 0.001; t = 7.882, P = 0.001), respectively; The mean MVD (41.02 +/- 8.55)/0.72 mm(2) of the integrin beta3 mRNA positive expression group was significantly higher than (25.26 +/- 11.25)/0.72 mm(2) of the negative expression group. There was a positive relation between MVD and integrin beta3 mRNA expression in the tumor (r(s) = 0.316, P = 0.001). The mean survival time in cases with positive integrin beta3 mRNA expression or MVD value >or= 39.5 was significantly shorter than that in cases with negative expression or MVD value < 39.5.
Integrin beta3 expression correlates with enhanced tumor angiogenesis and may play a role in the invasion and nodal metastasis of gastric carcinoma, therefore it may serve as a prognostic marker of gastric cancer.
探讨整合素β3在胃癌中的mRNA表达及其与微血管密度（MVD）、生长方式、侵袭、转移和预后的关系。
采用原位杂交和免疫组织化学技术研究105例胃癌标本中整合素β3 mRNA和CD34蛋白的表达。
原位杂交显示，非肿瘤胃黏膜中整合素β3 mRNA阳性率为30%（6/20），显著低于胃癌组（61.0%，64/105，χ² = 8.85，P = 0.037）；浸润型病例（70.2%，40/57）、Ⅲ - Ⅳ期（72.1%，44/61）、淋巴结转移（68.6%，48/70）和远处转移（85.7%，36/42）中，阳性表达率显著高于膨胀型（χ² = 14.97，P = 0.002）、Ⅰ - Ⅱ期（χ² = 15.21，P = 0.015）、无淋巴结转移（χ² = 17.89，P = 0.025）和无远处转移（χ² = 20.22，P = 0.005；χ² = 21.35，P = 0.035）；其平均MVD也显著高于膨胀型（t = 10.105，P = 0.001）、Ⅰ ～ Ⅱ期（t = 5.961，P = 0.001）、无淋巴结转移（t = 3.819，P = 0.01）和无远处转移（t = 10.578，P = 0.001；t = 7.882，P = 0.001）；整合素β3 mRNA阳性表达组的平均MVD（41.02±8.55）/0.72 mm²显著高于阴性表达组的（25.26±11.25）/0.72 mm²。肿瘤中MVD与整合素β3 mRNA表达呈正相关（r(s) = 0.316，P = 0.001）。整合素β3 mRNA表达阳性或MVD值≥39.5的病例平均生存时间显著短于表达阴性或MVD值＜39.5的病例。
整合素β3表达与肿瘤血管生成增强相关，可能在胃癌的侵袭和淋巴结转移中起作用，因此可作为胃癌的预后标志物。
Zotero 插件