Yoshida Ken-Ichi, Yamaguchi Masanori, Ikeda Hideki, Omae Kaoru, Tsurusaki Ken-Ichi, Fujita Yasutaro
Department of Biotechnology, Faculty of Life Science and Biotechnology, Fukuyama University, 985 Sanzo, Higashimura-cho, Fukuyama-shi, Hiroshima 729-0292, Japan.
Central Research Laboratories, Hokko Chemical Industry Co., Ltd, 2165 Toda, Atsugi-shi, Kanagawa 243-0023, Japan.
Microbiology (Reading). 2004 Mar;150(Pt 3):571-580. doi: 10.1099/mic.0.26768-0.
The myo-inositol catabolism pathway of Bacillus subtilis has not been fully characterized but was proposed to involve step-wise multiple reactions that finally yielded acetyl-CoA and dihydroxyacetone phosphate. It is known that the iolABCDEFGHIJ operon is responsible for the catabolism of inositol. IolG catalyses the first step of myo-inositol catabolism, the dehydrogenation of myo-inositol, producing 2-keto-myo-inositol (inosose). The second step was thought to be the dehydration of inosose. Genetic and biochemical analyses of the iol genes led to the identification of iolE, encoding the enzyme for the second step of inositol catabolism, inosose dehydratase. The reaction product of inosose dehydratase was identified as D-2,3-diketo-4-deoxy-epi-inositol.
枯草芽孢杆菌的肌醇分解代谢途径尚未完全明确，但据推测涉及一系列逐步反应，最终产生乙酰辅酶A和磷酸二羟丙酮。已知iolABCDEFGHIJ操纵子负责肌醇的分解代谢。IolG催化肌醇分解代谢的第一步，即肌醇脱氢，生成2-酮基-肌醇（肌糖）。第二步被认为是肌糖脱水。对iol基因的遗传和生化分析导致鉴定出iolE，它编码肌醇分解代谢第二步的酶——肌糖脱水酶。肌糖脱水酶的反应产物被鉴定为D-2,3-二酮-4-脱氧-表肌醇。
