菊苣果聚糖1-外切水解酶IIa的结晶及初步X射线衍射研究

Crystallization and preliminary X-ray diffraction study of fructan 1-exohydrolase IIa from Cichorium intybus.

作者信息

Verhaest Maureen, Van den Ende Wim, Yoshida Midori, Le Roy Katrien, Peeraer Yves, Sansen Stefaan, De Ranter Camiel J, Van Laere André, Rabijns Anja

机构信息

Laboratorium Voor Analytische Chemie en Medicinale Fysicochemie, Faculteit Farmaceutische Wetenschappen, K.U. Leuven, E. Van Evenstraat 4, B-3000 Leuven, Belgium.

出版信息

Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):553-4. doi: 10.1107/S0907444903029317. Epub 2004 Feb 25.

DOI:10.1107/S0907444903029317

PMID:14993690

Abstract

Fructan 1-exohydrolase IIa (1-FEH IIa), a plant enzyme involved in fructan breakdown, has been crystallized using the hanging-drop vapour-diffusion method at 277 K. The crystals are tetragonal, belonging to space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = 139.83, b = 139.83, c = 181.94 A. Calculation of the Matthews coefficient indicates there to be two or three molecules in the asymmetric unit. Synchrotron radiation was used to collect a complete native data set to a resolution of 2.35 A.

摘要

果聚糖1-外切水解酶IIa（1-FEH IIa）是一种参与果聚糖分解的植物酶，已采用悬滴气相扩散法在277 K下进行了结晶。晶体为四方晶系，属于空间群P4(1)2(1)2或P4(3)2(1)2，晶胞参数a = 139.83，b = 139.83，c = 181.94 Å。马修斯系数的计算表明，不对称单元中有两个或三个分子。使用同步辐射收集了完整的天然数据集，分辨率达到2.35 Å。