Furukawa S, Watanabe T, Tai T, Hirata J, Narisawa N, Kawarai T, Ogihara H, Yamasaki M
Laboratory of Food Microbiology, Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, 1866, Kameino, Fujisawa 252-8510, Japan.
Int J Food Microbiol. 2004 Mar 1;91(2):209-13. doi: 10.1016/S0168-1605(03)00372-6.
Effect of high pressure gaseous carbon dioxide treatment (HGCT) at 6.5 MPa, 35 degrees C on the germination of bacterial spores was investigated. Germination of bacterial spores was estimated by the decrease of heat tolerance. Approximately, 40% of Bacillus coagulans and 70% of Bacillus licheniformis were germinated by HGCT for 120 min at 35 degrees C, respectively. Germination was confirmed by phase contrast microscopy. The effect of hydrostatic pressure treatment (HPT) at 6.5 MPa, 35 degrees C on the germination of B. coagulans and B. licheniformis spores were also investigated. Spores did not germinate by HPT alone at 6.5 MPa for 120 min.
研究了在6.5兆帕、35摄氏度下高压气态二氧化碳处理(HGCT)对细菌芽孢萌发的影响。通过耐热性的降低来估计细菌芽孢的萌发情况。在35摄氏度下,HGCT处理120分钟后,分别约有40%的凝结芽孢杆菌和70%的地衣芽孢杆菌芽孢萌发。通过相差显微镜确认了芽孢的萌发。还研究了在6.5兆帕、35摄氏度下静水压力处理(HPT)对凝结芽孢杆菌和地衣芽孢杆菌芽孢萌发的影响。在6.5兆帕下单独进行HPT处理120分钟,芽孢不会萌发。