Krilov Dubravka, Stojanović Natasa, Herak Janko N
Medical School, University of Zagreb, Salata 3b, 10000 Zagreb, Croatia.
Chem Phys Lipids. 2004 Apr;129(1):75-84. doi: 10.1016/j.chemphyslip.2003.12.002.
Theoretical probabilistic kinetic model has been applied to describe the measurements of several oxidation markers as a function of time, during slow oxidation of low-density lipoprotein (LDL). It has been demonstrated that such a process could be described as tocopherol-mediated peroxidation (TMP), initiated and sustained by the action of copper ions, present in LDL in trace amounts. In that process concentration of alpha-tocopherol remains essentially unaltered. Tocopherol and copper ions act as catalysts, oscillating between the oxidized and reduced states. The fitting of the theoretical model to the experimental data resulted in determination of the numerical values for the kinetic parameters. It has been found that the parameter values used for the fitting of the data collected for a number of samples from various donors differ rather little. The kinetic chain length of 1.3 (in presence of co-antioxidants) and 2.9 (in the absence of co-antioxidants) is shorter than found by others. The difference probably comes from the much lower concentration of copper ions in our systems (about 0.1 ion per LDL particle).
理论概率动力学模型已被用于描述在低密度脂蛋白（LDL）缓慢氧化过程中几种氧化标志物随时间的测量结果。已经证明，这样一个过程可以描述为生育酚介导的过氧化作用（TMP），由痕量存在于LDL中的铜离子的作用引发并维持。在这个过程中，α-生育酚的浓度基本保持不变。生育酚和铜离子作为催化剂，在氧化态和还原态之间振荡。理论模型与实验数据的拟合确定了动力学参数的数值。已经发现，用于拟合从不同供体采集的多个样本数据的参数值差异很小。1.3（在存在共抗氧化剂的情况下）和2.9（在不存在共抗氧化剂的情况下）的动力学链长比其他人发现的要短。这种差异可能源于我们系统中铜离子的浓度低得多（每LDL颗粒约0.1个离子）。
