饮食和摄食状态对东亚飞蝗肠道中FMRF酰胺相关肽的影响

The influence of diet and feeding state on FMRFamide-related peptides in the gut of Locusta migratoria L.

作者信息

Hill Sharon R, Orchard Ian

机构信息

Department of Biology, University of Toronto at Mississauga, 3359 Mississauga Road North, Mississauga, Ont., Canada L5L 1C6.

出版信息

Peptides. 2004 Jan;25(1):105-14. doi: 10.1016/j.peptides.2003.12.007.

DOI:10.1016/j.peptides.2003.12.007

PMID:15003362

Abstract

Gut tissues of 2-week post-ecdysis female Locusta migratoria L. were assayed for FMRFamide-like immunoreactivity (FLI) during various feeding states using both radioimmunoassay and immunohistochemistry. The feeding states investigated were: (a) 48- and 24-h starved; (b) 5-, 30-, or 60-min post-feeding initiation; and (c) a diet of wheat grass, carrots, or apples. We determined: (1) the feeding state of a locust influences FLI in all gut tissues; (2) variations in diet appear to influence FLI in all gut tissues; (3) more than one FMRFamide-related peptide (FaRP) responds to differences in diet and state of starvation in the gut tissues; and (4) the protein poor diets (carrot and apple), in conjunction with the assertion that protein to carbohydrate ratio in the diet is the key component for nutrient balancing, suggests that FaRPs may play a role in maintaining balanced nutrient content in the locust.

摘要

利用放射免疫分析法和免疫组织化学方法，对羽化后2周的雌性飞蝗肠道组织在不同摄食状态下的FMRF酰胺样免疫反应性（FLI）进行了检测。所研究的摄食状态包括：（a）饥饿48小时和24小时；（b）开始进食后5分钟、30分钟或60分钟；（c）以小麦草、胡萝卜或苹果为食。我们确定：（1）蝗虫的摄食状态会影响所有肠道组织中的FLI；（2）饮食变化似乎会影响所有肠道组织中的FLI；（3）不止一种FMRF酰胺相关肽（FaRP）对肠道组织中饮食和饥饿状态的差异有反应；（4）蛋白质含量低的饮食（胡萝卜和苹果），再结合饮食中蛋白质与碳水化合物的比例是营养平衡关键成分这一观点，表明FaRPs可能在维持蝗虫营养成分平衡中发挥作用。