Xu D-H, Klesius P H, Shelby R A
Aquatic Animal Health Research Laboratory, Agricultural Research Service, US Department of Agriculture, Auburn, AL 36831, USA.
J Fish Dis. 2004 Mar;27(3):135-41. doi: 10.1111/j.1365-2761.2004.00523.x.
The humoral immune responses and host protection of channel catfish, Ictalurus punctatus (Rafinesque), against Ichthyophthirius multifiliis (Ich) were determined after immunization with live theronts and sonicated trophonts. Immunizations with live theronts or sonicated trophonts were carried out by both bath immersion and intraperitoneal (i.p.) injection. Cutaneous and serum immunoglobulin (Ig) levels and anti-Ich antibodies were measured 12 and 21 days post-immunization. The level of Ich infection and survival of catfish were determined after theront challenge. Cutaneous and serum anti-Ich antibodies were significantly higher (P < 0.05) in fish immunized with live theronts by immersion or i.p. injection, or with sonicated trophonts administered by i.p. injection, than in fish immunized with sonicated trophonts by immersion, with bovine serum albumin by i.p. injection, or non-immunized controls. Host protection was noted only in fish immunized with live theronts by immersion or i.p. injection or with sonicated trophonts by i.p. injection. There was a positive correlation between higher levels of anti-Ich antibodies and host survival in the immunized fish.
在用活的游动孢子和超声破碎的包囊体免疫斑点叉尾鮰（Ictalurus punctatus，拉芬斯克命名）后，测定了其针对多子小瓜虫（Ich）的体液免疫反应和宿主保护作用。通过浸浴和腹腔内（i.p.）注射两种方式，用活的游动孢子或超声破碎的包囊体进行免疫。在免疫后12天和21天，测量皮肤和血清免疫球蛋白（Ig）水平以及抗小瓜虫抗体。在游动孢子攻击后，测定小瓜虫感染水平和鲶鱼的存活率。通过浸浴或腹腔注射用活的游动孢子免疫的鱼，或通过腹腔注射用超声破碎的包囊体免疫的鱼，其皮肤和血清抗小瓜虫抗体显著高于（P < 0.05）通过浸浴用超声破碎的包囊体免疫的鱼、通过腹腔注射用牛血清白蛋白免疫的鱼或未免疫的对照鱼。仅在通过浸浴或腹腔注射用活的游动孢子免疫的鱼，或通过腹腔注射用超声破碎的包囊体免疫的鱼中观察到宿主保护作用。在免疫的鱼中，较高水平的抗小瓜虫抗体与宿主存活率之间存在正相关。
