Dimerization of recombinant tobacco mosaic virus movement protein.
作者信息
Brill Laurence M, Dechongkit Songpon, DeLaBarre Byron, Stroebel Jonathon, Beachy Roger N, Yeager Mark
机构信息
Department of Cell Biology, The Scripps Research Institute, Scripps Clinic, La Jolla, California 92037, USA.
出版信息
J Virol. 2004 Apr;78(7):3372-7. doi: 10.1128/jvi.78.5.3372-3377.2004.
Abstract
The p30 movement protein (MP) is essential for cell-to-cell spread of tobacco mosaic virus in planta. We used anion-exchange chromatography and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to obtain highly purified 30-kDa MP, which migrated as a single band in native PAGE. Analytical ultracentrifugation suggested that the protein was monodisperse and dimeric in the nonionic detergent n-octyl-beta-D-glucopyranoside. Circular dichroism (CD) spectroscopy showed that the detergent-solubilized protein contained significant alpha-helical secondary structure. Proteolysis of the C-tail generated a trypsin-resistant core that was a mixture of primarily monomers and some dimers. We propose that MP dimers are stabilized by electrostatic interactions in the C terminus as well as hydrophobic interactions between putative transmembrane alpha-helical coiled coils.
摘要
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本文引用的文献
1
The 30-kilodalton gene product of tobacco mosaic virus potentiates virus movement.
Science. 1987 Jul 24;237(4813):389-94. doi: 10.1126/science.237.4813.389.
2
Movement protein of tobacco mosaic virus modifies plasmodesmatal size exclusion limit.
Science. 1989 Oct 20;246(4928):377-9. doi: 10.1126/science.246.4928.377.
3
Function of the 30 kd protein of tobacco mosaic virus: involvement in cell-to-cell movement and dispensability for replication.
EMBO J. 1987 Sep;6(9):2557-63. doi: 10.1002/j.1460-2075.1987.tb02544.x.
4
Persistence of native-like topology in a denatured protein in 8 M urea.
Science. 2001 Jul 20;293(5529):487-9. doi: 10.1126/science.1060438.
5
Plasmodesmata and plant cytoskeleton.
Trends Plant Sci. 2001 Jul;6(7):326-30. doi: 10.1016/s1360-1385(01)01981-1.
6
Function of microtubules in intercellular transport of plant virus RNA.
Nat Cell Biol. 2000 Nov;2(11):826-32. doi: 10.1038/35041072.
7
Role of microtubules in the intracellular distribution of tobacco mosaic virus movement protein.
Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):12345-9. doi: 10.1073/pnas.97.22.12345.
8
Regulation of plasmodesmal transport by phosphorylation of tobacco mosaic virus cell-to-cell movement protein.
EMBO J. 2000 Sep 15;19(18):4875-84. doi: 10.1093/emboj/19.18.4875.
9
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10
A method for directly fitting the time derivative of sedimentation velocity data and an alternative algorithm for calculating sedimentation coefficient distribution functions.
Anal Biochem. 2000 Mar 15;279(2):151-63. doi: 10.1006/abio.2000.4480.
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