哺乳动物眼睛中犬尿喹啉酸的含量及犬尿氨酸转氨酶的活性

Content of kynurenic acid and activity of kynurenine aminotransferases in mammalian eyes.

作者信息

Zarnowski Tomasz, Rejdak Robert, Zagorski Zbigniew, Juenemann Anselm G M, Zrenner Eberhart, Kocki Tomasz, Urbanska Ewa M, Turski Waldemar A

机构信息

Tadeusz Krwawicz Chair of Ophthalmology and 1st Eye Hospital, Lublin, Poland.

出版信息

Ophthalmic Res. 2004 Mar-Apr;36(2):124-8. doi: 10.1159/000076893.

DOI:10.1159/000076893

PMID:15017110

Abstract

The present study investigated the kynurenic acid (KYNA) contents and kynurenine aminotransferase (KAT I and II) activity in structures of the human, monkey, rabbit and bovine eye. KYNA levels were investigated with HPLC and detected fluorimetrically. The activity of KAT I and II was assayed as quantitative analysis of newly synthesized KYNA in vitro. Mean KYNA levels (+/-SD) in the human retina and vitreous body were 36.8 +/- 7.6 and 33.1 +/- 6.2 pmol/g wet tissue weight, respectively. In human eyes, KAT I activity in the vitreous body was 0.57 +/- 0.28, that of KAT II was 2.56 +/- 0.69. KAT I activity in the retina was 3.42 +/- 1.17 and that of KAT II 10.75 +/- 9.2. (KAT activity is expressed as KYNA synthesis in picomoles per gram wet tissue weight per hour.) The values of KYNA and KAT observed in other mammalian species tested were in the same range. In conclusion, KYNA and KAT enzymatic activity are present in the structures of human and other mammalian eyes.

摘要

本研究调查了人、猴、兔和牛眼结构中的犬尿喹啉酸（KYNA）含量以及犬尿氨酸转氨酶（KAT I和II）活性。采用高效液相色谱法（HPLC）并通过荧光检测法来研究KYNA水平。KAT I和II的活性通过对体外新合成的KYNA进行定量分析来测定。人视网膜和玻璃体中KYNA的平均水平（±标准差）分别为36.8±7.6和33.1±6.2皮摩尔/克湿组织重量。在人眼中，玻璃体中KAT I的活性为0.57±0.28，KAT II的活性为2.56±0.69。视网膜中KAT I的活性为3.42±1.17，KAT II的活性为10.75±9.2。（KAT活性以每克湿组织重量每小时合成的KYNA皮摩尔数表示。）在其他受试哺乳动物物种中观察到的KYNA和KAT值处于相同范围内。总之，KYNA和KAT酶活性存在于人和其他哺乳动物的眼部结构中。