编码一种缺陷型受体蛋白酪氨酸激酶的全长小鼠Ptk7 cDNA的克隆与鉴定。

Cloning and characterization of the full-length mouse Ptk7 cDNA encoding a defective receptor protein tyrosine kinase.

作者信息

Jung Jae-Won, Shin Won-Sik, Song Jihwan, Lee Seung-Taek

机构信息

Department of Biochemistry, College of Science, and Protein Network Research Center, Yonsei University, Seoul 120-749, South Korea.

出版信息

Gene. 2004 Mar 17;328:75-84. doi: 10.1016/j.gene.2003.12.006.

DOI:10.1016/j.gene.2003.12.006

PMID:15019986

Abstract

The 3.8-kb full-length mouse Ptk7 cDNA encoding a defective receptor protein tyrosine kinase was cloned by reverse transcription-PCR of mouse liver mRNA. The mouse PTK7 polypeptide showed 92.6% identity to human PTK7. The mouse Ptk7 gene consists of 20 exons and has exactly the same exon structure as the human PTK7 gene. Mouse PTK7 was shown to be phosphorylated neither by itself nor by other protein tyrosine kinases. In addition, its expression did not affect the phospho-tyrosine level of cellular proteins in COS-1 cells. The mouse Ptk7 mRNA was expressed at high levels in lung and un-pregnant uterus among adult tissues, and in the tail, limbs, somites, gut, and craniofacial regions among embryonic tissues. These data suggest that mouse PTK7, an orthologue of human PTK7, plays multiple roles in embryonic development.

摘要

通过对小鼠肝脏mRNA进行逆转录聚合酶链反应（RT-PCR），克隆出了编码缺陷型受体蛋白酪氨酸激酶的3.8 kb全长小鼠Ptk7 cDNA。小鼠PTK7多肽与人类PTK7的同一性为92.6%。小鼠Ptk7基因由20个外显子组成，其外显子结构与人类PTK7基因完全相同。研究表明，小鼠PTK7既不能自身磷酸化，也不能被其他蛋白酪氨酸激酶磷酸化。此外，其表达不影响COS-1细胞中细胞蛋白的磷酸酪氨酸水平。小鼠Ptk7 mRNA在成年组织的肺和未孕子宫中高表达，在胚胎组织的尾部、四肢、体节、肠道和颅面部区域也高表达。这些数据表明，小鼠PTK7作为人类PTK7的直系同源物，在胚胎发育中发挥多种作用。