流动量热法——一种用于测定来自酒石酸诺卡氏菌的固定化顺式环氧琥珀酸水解酶活性的有用工具。
Flow calorimetry--a useful tool for determination of immobilized cis-epoxysuccinate hydrolase activity from Nocardia tartaricans.
作者信息
Vikartovská Alica, Bucko Marek, Gemeiner Peter, Nahálka Jozef, Pätoprstý Vladimir, Hrabárová Eva
机构信息
Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovak Republic.
出版信息
Artif Cells Blood Substit Immobil Biotechnol. 2004 Feb;32(1):77-89. doi: 10.1081/bio-120028670.
Bacterial cells Nocardia tartaricans with cis-epoxysuccinate hydrolase activity were entrapped in hardened calcium pectate gel by a commercial high performance encapsulator. This enzyme (in a single step reaction with no formation of side products) was used to hydrolyze disodium cis-epoxysuccinate to a pure enantiomer--disodium L-(+)-tartrate. Activities of this enzyme were determined using flow calorimetry. The validity of this method was corroborated by HPLC and isotachophoresis. The immobilized biocatalyst has activity (75.8 U/mgdry) able to convert disodium cis-epoxysuccinate to disodium tartrate at 94% yield in 5.5h. Immobilization of N. tartaricans in hardened calcium pectate gel beads had a positive effect on the activity of cis-epoxysuccinate hydrolase, storage stability, yield, and time of bioconversion.
具有顺式环氧琥珀酸水解酶活性的诺卡氏菌（Nocardia tartaricans）细菌细胞被一种商用高效包囊机包埋在硬化的果胶酸钙凝胶中。该酶（在无副产物形成的单步反应中）用于将顺式环氧琥珀酸钠水解为纯对映体——L-(+)-酒石酸钠二钠。使用流动量热法测定该酶的活性。通过高效液相色谱法（HPLC）和等速电泳法证实了该方法的有效性。固定化生物催化剂具有活性（75.8 U/mgdry），能够在5.5小时内以94%的产率将顺式环氧琥珀酸钠转化为酒石酸钠二钠。将诺卡氏菌包埋在硬化的果胶酸钙凝胶珠中对顺式环氧琥珀酸水解酶的活性、储存稳定性、产率和生物转化时间具有积极影响。
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