Time-course evaluation of whole blood phagocytosis in mice treated with the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine.

Immunological abnormalities have been described in idiopathic Parkinson's disease and in the mouse model of this disorder induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). This investigation was aimed to study the effect of MPTP on inflammatory response of whole blood phagocytes at different time intervals. C57BL male mice were injected intraperitoneally with either MPTP (30 mg/kg) or saline (control group) and the blood samples were collected at 4, 24 and 48 h. 50 microl of a 500-fold diluted blood sample was mixed with 150 microl of reaction mixture (0.4 mM luminol + 50 microg opsonized zymosan + 0.1% gelatin, in Hanks' balanced salt solution) and the chemiluminescence (CL) signal was measured in a luminometer at 37 degrees C. Although the CL response of the whole blood from control and MPTP groups was similar at 4 h, a significant increase in the CL signal was observed in MPTP-treated mice at 24 h post-treatment, which got subsided at 48 h. The findings of this study suggest that in an early stage a pro-inflammatory response following MPTP might trigger a chain of potentially toxic pathways mediated by reactive oxygen species, leading to progressive neuronal damage.