碳酸酐酶在兔回肠肠上皮细胞和Caco-2细胞中的亚细胞分布。存在两种与刷状缘膜结合的同工酶的证据。
Subcellular distribution of carbonic anhydrase in the enterocyte of the rabbit ileum and the Caco-2 cell. Evidence for the presence of two isozymes bound to brush-border membranes.
作者信息
Aguenaou H, Colin D A
机构信息
Laboratoire de Toxinologie Bactérienne et des Agents Anti-infectieux, Faculté de Médecine, Université Louis Pasteur, Strasbourg, France.
出版信息
Biochim Biophys Acta. 1992 Aug 10;1109(1):59-64. doi: 10.1016/0005-2736(92)90187-q.
The activity of CA has been determined in the membranes of enterocytes from rabbit ileum and of Caco-2 cells. No CA activity was detected in the BLM, but the activity in the BBM (43 and 7 WAU/mg protein for rabbit and Caco-2, respectively) was doubled by the addition of Triton. These two types of activity could be distinguished in rabbit ileum by their different IC50 in the presence of acetazolamide (10(-5) and 5 x 10(-7) M) and their different sensitivities to heat. They were not modified by inhibitors of cytoplasmic isozymes and seem to correspond to two forms of CA, one situated in the extracellular leaflet of the BBM and the other one in the intracellular leaflet.
已测定兔回肠肠上皮细胞和Caco - 2细胞的细胞膜中碳酸酐酶（CA）的活性。在基底外侧膜（BLM）中未检测到CA活性，但添加 Triton后，刷状缘膜（BBM）中的活性（兔和Caco - 2分别为43和7 WAU/mg蛋白）增加了一倍。在兔回肠中，这两种类型的活性可通过它们在乙酰唑胺存在下不同的半数抑制浓度（IC50）（分别为10⁻⁵和5×10⁻⁷ M）以及对热的不同敏感性来区分。它们不受细胞质同工酶抑制剂的影响，似乎对应于两种形式的CA，一种位于BBM的细胞外小叶，另一种位于细胞内小叶。
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