Khoruts Alexander, Osness Richard E, Jenkins Marc K
Department of Medicine, Center for Immunology, University of Minnesota Medical School, Minneapolis, 55455, USA.
Eur J Immunol. 2004 Apr;34(4):1085-90. doi: 10.1002/eji.200324170.
The basis of the adjuvant effect of IL-1 on the clonal expansion of Ag-specific CD4 T cells was measured in vivo using the TCR transgenic T cell adoptive transfer method. Injection of Ag plus IL-1 caused naive CD4 T cells to proliferate to a greater extent than did injection of Ag alone. The T cells had to express CD28 to experience the beneficial effect of IL-1 whereas the host had to express the type I IL-1R, raising the possibility that IL-1's adjuvant properties were related to induction of CD28 ligands on APC. Surprisingly, however, expression of CD28 ligands on DC and B cells was not affected by IL-1. Therefore, the adjuvant properties of IL-1 depend on a basal level of CD28 signaling but cannot be explained by enhanced CD28 signaling due to CD28 ligand induction.
利用TCR转基因T细胞过继转移方法在体内检测了白细胞介素-1（IL-1）对抗原特异性CD4 T细胞克隆扩增的佐剂效应基础。注射抗原加IL-1比单独注射抗原能使幼稚CD4 T细胞增殖得更多。T细胞必须表达CD28才能体验到IL-1的有益作用，而宿主必须表达I型IL-1受体，这增加了IL-1的佐剂特性与在抗原递呈细胞（APC）上诱导CD28配体有关的可能性。然而，令人惊讶的是，DC和B细胞上CD28配体的表达不受IL-1影响。因此，IL-1的佐剂特性取决于CD28信号的基础水平，但不能用因CD28配体诱导导致的CD28信号增强来解释。
