Jamsari A, Nitz I, Reamon-Büttner S M, Jung C
Plant Breeding Institute, Christian-Albrechts-University of Kiel, Olshausenstrasse 40, 24098, Kiel, Germany.
Theor Appl Genet. 2004 Apr;108(6):1140-6. doi: 10.1007/s00122-003-1529-0. Epub 2003 Dec 13.
A HindIII BAC (bacterial artificial chromosome) library of asparagus ( Asparagus officinalis L.) was established from a single male plant homozygous for the male flowering gene ( MM). The library represents approximately 5.5 haploid genome equivalents with an average insert size of 82 kb. A subset of the library (2.6 haploid genome equivalents) was arranged into DNA pools. Using nine sex-linked amplified fragment length polymorphism (AFLP) and two sequence-tagged site (STS) markers, 13 different BAC clones were identified from this part of the library. The BACs were arranged into a first-generation physical map around the sex locus. Four PCR-derived markers were developed from the BAC ends, one of which could be scored in a co-dominant way. Using a mapping population of 802 plants we mapped the BAC-derived markers to the same position close to the M gene as the corresponding AFLP and STS markers. The markers are useful for further chromosome walking studies and as diagnostic markers for selecting male plants homozygous for the M gene.
从一株携带雄性开花基因（MM）纯合的雄性芦笋（Asparagus officinalis L.）植株构建了一个芦笋的HindIII细菌人工染色体（BAC）文库。该文库约相当于5.5个单倍体基因组，平均插入片段大小为82 kb。文库的一个子集（相当于2.6个单倍体基因组）被排列成DNA池。利用9个性别连锁扩增片段长度多态性（AFLP）标记和2个序列标签位点（STS）标记，从文库的这一部分鉴定出13个不同的BAC克隆。这些BAC被排列成围绕性别位点的第一代物理图谱。从BAC末端开发了4个PCR衍生标记，其中一个可以共显性方式进行评分。利用一个由802株植物组成的作图群体，我们将BAC衍生标记定位到与相应AFLP和STS标记相同的靠近M基因的位置。这些标记对于进一步的染色体步移研究很有用，并且可作为诊断标记用于选择携带M基因纯合的雄性植株。
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