使用小鼠脑突触神经小体对麻痹性贝类毒素进行定量分析。

Quantitation of paralytic shellfish toxins using mouse brain synaptoneurosomes.

作者信息

David Laurence S, Nicholson Russell A

机构信息

Department of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, British Columbia, Canada V5A 1S6.

出版信息

Chemosphere. 2004 Jun;55(10):1315-21. doi: 10.1016/j.chemosphere.2003.10.056.

DOI:10.1016/j.chemosphere.2003.10.056

PMID:15081774

Abstract

A membrane potential assay based on synaptoneurosomes prepared from mouse brain was evaluated further for its utility in estimating saxitoxin and related bioactives. Saxitoxin concentrations quantitated in mussel extracts by the synaptoneurosomal technique correlated well with spiked concentrations in these samples (r2 = 0.995; slope=1.048). Other experiments found that the synaptoneurosomal assay can detect saxitoxin-like bioactives in zooplankton samples and the concentrations measured were consistent with preliminary estimations of saxitoxin equivalents using the [3H] saxitoxin receptor binding technique. Veratrine, a mixture of alkaloids that activate sodium channels, had similar potential as a substitute for veratridine in the synaptoneurosomal assay. The results provide additional evidence that the mouse brain synaptoneurosomal membrane potential assay has excellent capability for quantitation of saxitoxin-like activity in shellfish tissues and may also be applied to zooplankton samples.

摘要

基于从小鼠脑制备的突触体进行的膜电位测定，进一步评估了其在估计石房蛤毒素及相关生物活性物质方面的效用。通过突触体技术对贻贝提取物中石房蛤毒素浓度进行定量，与这些样品中的加标浓度相关性良好（r2 = 0.995；斜率 = 1.048）。其他实验发现，突触体测定可检测浮游动物样品中的石房蛤毒素样生物活性物质，且所测浓度与使用[3H]石房蛤毒素受体结合技术对石房蛤毒素当量的初步估计一致。藜芦碱是一种激活钠通道的生物碱混合物，在突触体测定中作为藜芦定的替代品具有相似潜力。结果提供了更多证据，表明小鼠脑突触体膜电位测定在定量贝类组织中石房蛤毒素样活性方面具有出色能力，也可应用于浮游动物样品。