用于在水合磷脂双层中定向的肽样品的交叉极化方案。
Cross-polarization schemes for peptide samples oriented in hydrated phospholipid bilayers.
作者信息
Kim Hyeongnam, Cross Timothy A, Fu Riqiang
机构信息
Center for Interdisciplinary Magnetic Resonance, National High Magnetic Field Laboratory, and Department of Chemistry and Biochemistry, Florida State University, Tallahassee, USA.
出版信息
J Magn Reson. 2004 May;168(1):147-52. doi: 10.1016/j.jmr.2004.02.010.
Continuous-wave, ramped amplitude, and frequency modulated cross-polarization schemes (abbreviated as CWCP, RACP, and FMCP, respectively) are evaluated for static samples in anisotropic phases, such as peptides oriented in lipid environments. It is shown experimentally that both RACP and FMCP give rise to 20% higher polarized signal intensity in comparison to CWCP. The CP matching bandwidths for CWCP and RACP are about the same. Because of its adiabaticity, FMCP has a much broader CP matching bandwidth than CWCP and RACP. In addition, the (15)N RF amplitude used at the center of the FMCP matching profile is much lower than that of the CWCP and RACP matching profiles. A sample of [(15)N]Leu(4) labeled gramicidin A oriented in lipid bilayers was used to demonstrate these experiments.
针对各向异性相中的静态样品，如脂质环境中取向的肽，评估了连续波、斜坡幅度和频率调制交叉极化方案（分别简称为CWCP、RACP和FMCP）。实验表明，与CWCP相比，RACP和FMCP均能产生高20%的极化信号强度。CWCP和RACP的交叉极化匹配带宽大致相同。由于其绝热性，FMCP的交叉极化匹配带宽比CWCP和RACP宽得多。此外，FMCP匹配曲线中心处使用的(15)N射频幅度远低于CWCP和RACP匹配曲线。使用脂质双层中取向的[(15)N]Leu(4)标记短杆菌肽A样品来演示这些实验。
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