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一种用于免疫传感生物芯片的基于聚合物的微流控装置。

A polymer-based microfluidic device for immunosensing biochips.

作者信息

Soo Ko Jong, Yoon Hyun C, Yang Haesik, Pyo Hyeon-Bong, Hyo Chung Kwang, Jin Kim Sung, Tae Kim Youn

机构信息

BioMEMS Group, Basic Research Laboratory, ETRI, Daejeon 305600, Korea.

出版信息

Lab Chip. 2003 May;3(2):106-13. doi: 10.1039/b301794j. Epub 2003 Apr 30.

Abstract

This paper describes the design, fabrication, and test of a PDMS/PMMA-laminated microfluidic device for an immunosensing biochip. A poly(dimethyl siloxane)(PDMS) top substrate molded by polymer casting and a poly(methyl methacrylate)(PMMA) bottom substrate fabricated by hot embossing are bonded with pressure and hermetically sealed. Two inlet ports and an air vent are opened through the PDMS top substrate, while gold electrodes for electrochemical biosensing are patterned onto the PMMA bottom substrate. The analyte sample is loaded from the sample inlet port to the detection chamber by capillary force, without any external intervening forces. For this and to control the time duration of sample fluid in each compartment of the device, including the inlet port, diffusion barrier, reaction chamber, flow-delay neck, and detection chamber, the fluid conduit has been designed with various geometries of channel width, depth, and shape. Especially, the fluid path has been designed so that the sample flow naturally stops after filling the detection chamber to allow sufficient time for biochemical reaction and subsequent washing steps. As model immunosensing tests for the microfluidic device, functionalizations of ferritin and biotin to the sensing surfaces on gold electrodes and their biospecific interactions with antiferritin antiserum and streptavidin have been investigated. An electrochemical detection method for immunosensing by biocatalyzed precipitation has been developed and applied for signal registration. With the biochip, the whole immunosensing processes could be completed within 30 min.

摘要

本文描述了一种用于免疫传感生物芯片的聚二甲基硅氧烷(PDMS)/聚甲基丙烯酸甲酯(PMMA)层压微流控装置的设计、制造和测试。通过聚合物浇铸成型的聚二甲基硅氧烷(PDMS)顶部基板和通过热压花制造的聚甲基丙烯酸甲酯(PMMA)底部基板在压力作用下键合并气密密封。通过PDMS顶部基板开设两个入口端口和一个排气孔,而用于电化学生物传感的金电极则图案化在PMMA底部基板上。分析物样品通过毛细作用力从样品入口端口加载到检测室,无需任何外部干预力。为此并为了控制样品流体在装置的每个隔室(包括入口端口、扩散屏障、反应室、流动延迟颈部和检测室)中的持续时间,流体导管已设计成具有不同的通道宽度、深度和形状的几何结构。特别是,流体路径的设计使得样品流在充满检测室后自然停止,以便为生化反应和后续洗涤步骤留出足够的时间。作为对微流控装置的模型免疫传感测试,研究了铁蛋白和生物素在金电极传感表面上的功能化及其与抗铁蛋白抗血清和链霉亲和素的生物特异性相互作用。开发了一种通过生物催化沉淀进行免疫传感的电化学检测方法并将其应用于信号记录。使用该生物芯片,整个免疫传感过程可在30分钟内完成。

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