Identification of three novel alleles: DRB3*0110, DRB1*1140, and DRB1*140102.

Three novel human leukocyte antigen class II alleles (DRB30110, DRB11140, and DRB1140102) are described here. The three novel alleles were initially detected as previously unidentified SSO hybridization patterns using CANTYPE((R)) reverse hybridization assay. Sequences were determined by cloning/sequencing. DRB30110 allele is identical to DRB3010101, except for a single nucleotide substitution (CGC-->AGC) changing codon 39 from Arg to Ser. This polymorphism has not, until now, been identified in DRB allele. Thus, this is an unusual mutation as the codon 39 is a fairly conserved region. The new DRB11140 is identical to DRB11116, except for a single nucleotide substitution at codon 67 from ATC (encoding for isoleucine) to TTC (encoding for phenylalanine). This polymorphism is commonly found in DRB111 alleles. Compared with DRB1140101, DRB1140102 contains a single silent nucleotide substitution (TAT-->TAC, both encoding for tyrosine) at codon 78. This polymorphism is commonly found in DRB114 alleles. The three new DRB alleles may have been generated by a point mutation event. The DRB30110 and DRB1140102 were identified in Caucasoid individuals. The ethnic origin of the subject carrying the DRB11140 allele is Egyptian. The DRB1140102 was detected in two unrelated individuals; the DRB30110 and DRB1*1140 were only identified once, in a total population of 80,000.