铜绿假单胞菌抗菌药敏试验的快速比色测定法。
Rapid colorimetric assay for antimicrobial susceptibility testing of Pseudomonas aeruginosa.
作者信息
Tunney Michael M, Ramage Gordon, Field Tyler R, Moriarty Thomas F, Storey Douglas G
机构信息
The Clinical and Practice Research Group, School of Pharmacy, The Queen's University of Belfast, Belfast BT9 7BL, United Kingdom.
出版信息
Antimicrob Agents Chemother. 2004 May;48(5):1879-81. doi: 10.1128/AAC.48.5.1879-1881.2004.
Abstract
A colorimetric assay based on the reduction of a tetrazolium salt [2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT)] for rapidly determining the susceptibility of Pseudomonas aeruginosa isolates to bactericidal antibiotics is described. There was excellent agreement between the tobramycin and ofloxacin MICs determined after 5 h using the XTT assay and after 18 h using conventional methods. The data suggests that an XTT-based assay could provide a useful method for rapidly determining the susceptibility of P. aeruginosa to bactericidal antibiotics.
摘要
描述了一种基于四氮唑盐[2,3-双[2-甲氧基-4-硝基-5-磺基苯基]-2H-四氮唑-5-羧基苯胺（XTT）]还原的比色测定法，用于快速确定铜绿假单胞菌分离株对杀菌抗生素的敏感性。使用XTT测定法在5小时后和使用传统方法在18小时后测定的妥布霉素和氧氟沙星的最低抑菌浓度（MIC）之间具有极好的一致性。数据表明，基于XTT的测定法可为快速确定铜绿假单胞菌对杀菌抗生素的敏感性提供一种有用的方法。
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