Kotlyar A B, Gutman M, Ackrell B A
Dept. of Biochemistry, Tel Aviv University, Ramat Aviv, Israel.
Biochem Biophys Res Commun. 1992 Aug 14;186(3):1656-62. doi: 10.1016/s0006-291x(05)81598-0.
Two new methods have been devised for measuring fumarate reduction by beef heart succinate-ubiquinone oxidoreductase with quinols as electron donors. In one assay the quinone is maintained in the reduced state by coupling fumarate reduction with the DT-diaphorase reaction, in the other assay by the presence of excess dithionite. The advantages of these methods are discussed, along with preliminary characterization of the quinol-protein interaction.
已经设计出两种新方法，用于以喹醇作为电子供体，测量牛心琥珀酸 - 泛醌氧化还原酶催化的富马酸还原反应。在一种测定方法中，通过将富马酸还原与DT - 黄递酶反应偶联，使醌保持在还原状态；在另一种测定方法中，则通过加入过量连二亚硫酸盐来实现。文中讨论了这些方法的优点，以及对喹醇 - 蛋白质相互作用的初步表征。
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