肝片吸虫组织蛋白酶L1（FheCL1）基因cDNA的克隆与测序
[Cloning and sequencing of cathepsin L1 (FheCL1) gene cDNA of Fasciola hepatica].
作者信息
Zhang Ren, Li Hai-yun
机构信息
College of Animal Science, South China Agricultural University, Guangzhou 510642.
出版信息
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2003;21(5):282-5.
OBJECTIVE
To search for a candidate DNA vaccine of Fasciola hepatica.
METHODS
Using RT-PCR and digestion with Hind III and BamHI, Fasciola hepatica secreted cathepsin L1 (FheCL1) cDNA was cloned into the expression vector pcDNA3.1.
RESULTS
The cloning was successful, the cDNA sequence and its deduced amino acid sequence were analyzed. There was much difference between the cloned FheCL1 and the published one. But the first 20 residues of their amino acid sequences were the same.
CONCLUSION
The recombinant plasmid pcDNA3.1-FheCL1 may be a new type of candidate DNA vaccine candidate for Fasciola hepatica. It is possible that Fasciola hepatica presents different sub-species but their amino acid residues (1 to 20) encoded by FheCL1 might build up membrane spanning helix.
目的
寻找肝片吸虫的候选DNA疫苗。
方法
采用逆转录聚合酶链反应（RT-PCR）以及用 Hind III 和 BamHI 进行酶切，将肝片吸虫分泌的组织蛋白酶L1（FheCL1）cDNA 克隆到表达载体 pcDNA3.1 中。
结果
克隆成功，对 cDNA 序列及其推导的氨基酸序列进行了分析。克隆的 FheCL1 与已发表的序列有很大差异。但它们氨基酸序列的前20个残基相同。
结论
重组质粒 pcDNA3.1-FheCL1 可能是一种新型的肝片吸虫候选DNA疫苗。肝片吸虫可能存在不同亚种，但由 FheCL1 编码的氨基酸残基（1至20）可能形成跨膜螺旋。
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