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通过荧光相关光谱法探究巨型单层囊泡中的脂域形成与动力学。

Lipid domain formation and dynamics in giant unilamellar vesicles explored by fluorescence correlation spectroscopy.

作者信息

Kahya Nicoletta, Scherfeld Dag, Bacia Kirsten, Schwille Petra

机构信息

Experimental Biophysics Group, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Goettingen, Germany.

出版信息

J Struct Biol. 2004 Jul;147(1):77-89. doi: 10.1016/j.jsb.2003.09.021.

Abstract

Lipids in eukaryotic cell membranes have been shown to cluster in "rafts" with different lipid/protein compositions and molecular packing. Model membranes such as giant unilamellar vesicles (GUVs) provide a key system to elucidate the physical mechanisms of raft assembly. Despite the large amount of work devoted to the detection and characterization of rafts, one of the most important pieces of information still missing in the picture of the cell membrane is dynamics: how lipids organize and move in rafts and how they modulate membrane fluidity. This missing element is of crucial importance for the trafficking at and from the periphery of the cell regulated by endo- and exocytosis and, in general, for the constant turnover which redistributes membrane components. Here, we review studies of combined confocal fluorescence microscopy and fluorescence correlation spectroscopy on lipid dynamics and organization in rafts assembled in GUVs prepared from various lipid mixtures which are relevant to the problem of raft formation.

摘要

真核细胞膜中的脂质已被证明会聚集在具有不同脂质/蛋白质组成和分子排列的“筏”中。诸如巨型单层囊泡(GUVs)之类的模型膜提供了一个关键系统,以阐明筏组装的物理机制。尽管致力于筏的检测和表征的工作很多,但细胞膜图景中仍然缺失的最重要信息之一是动力学:脂质如何在筏中组织和移动,以及它们如何调节膜流动性。这个缺失的元素对于由内吞作用和外排作用调节的细胞周边的物质运输,以及一般而言对于重新分配膜成分的持续更新至关重要。在这里,我们回顾了结合共聚焦荧光显微镜和荧光相关光谱对由各种与筏形成问题相关的脂质混合物制备的GUVs中组装的筏内脂质动力学和组织的研究。

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