从产志贺毒素大肠杆菌O157:H7中鉴定出一种含有类IS1203元件的I型志贺毒素变体。

Identification of a Shiga-toxin type I variant containing an IS1203-like element, from Shiga-toxin producing Escherichia coli O157:H7.

作者信息

Suzuki Masahiro, Kondo Fumio, Ito Yuko, Matsumoto Masakado, Hata Mami, Oka Hisao, Takahashi Masao, Sakae Kenji

机构信息

Aichi Prefectural Institute of Public Health, 7-6 Nagare, Tsuji-machi, Kita-ku, Nagoya 462-8576, Japan.

出版信息

FEMS Microbiol Lett. 2004 May 1;234(1):63-7. doi: 10.1016/j.femsle.2004.03.008.

DOI:10.1016/j.femsle.2004.03.008

PMID:15109720

Abstract

We found two Shiga toxin producing Escherichia coli O157:H7 strains isolated from humans carrying the stx(1) gene with an IS1203-like element (designated as IS1203v(1)). The IS1203v(1) was inserted into the coding region of the A subunit 7 bp upstream from the TGA termination codon, resulting in a loss of two amino acid residues (Ser-Ser) from its C terminus. Toxicity of the Stx1 was confirmed by Vero cell assay. IS1203v(1) hardly affected the stx(1) gene in either its expression or the toxicity of its product.

摘要

我们发现从人类分离出的两株产志贺毒素大肠杆菌O157:H7菌株携带stx(1)基因及一个类IS1203元件（命名为IS1203v(1)）。IS1203v(1)插入到A亚基编码区TGA终止密码子上游7 bp处，导致其C末端缺失两个氨基酸残基（丝氨酸-丝氨酸）。通过Vero细胞试验证实了Stx1的毒性。IS1203v(1)对stx(1)基因的表达及其产物的毒性几乎没有影响。