具有药理活性的葡萄皮提取物的遗传毒性评估。

Genotoxic evaluation of a vinifera skin extract that present pharmacological activities.

作者信息

Aiub C, Stankevicins L, da Costa V, Ferreira F, Mazzei J, Ribeiro da Silva A, Soares de Moura R, Felzenszwalb I

机构信息

Departamento de Biofísica e Biometria e, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, 20551-030, Rio de Janeiro, Brazil.

出版信息

Food Chem Toxicol. 2004 Jun;42(6):969-73. doi: 10.1016/j.fct.2004.02.009.

DOI:10.1016/j.fct.2004.02.009

PMID:15110106

Abstract

Toxicity of an alcohol-free hydro-alcoholic grape skin extract (GSE) obtained from red grapes Vitis labrusca (Isabel varietal) that present antihypertensive, vasodilator and antioxidant effects was estimated by different bioassays. Using the Salmonella/microsome assay for strains TA97, TA98, TA100 and TA102 no mutagenicity was detected for all tested concentrations (0.1-100 microg/ml), even with metabolization. Nevertheless, cytotoxicity was observed for TA97 and TA102 with and without metabolization and for TA100 with metabolization. The measurement of beta-galactosidase induction in the SOS-chromotest was positive only for Escherichia coli PQ37 when metabolization enzymes were present. Using Balb/c 3T3 fibroblasts, DNA strand breaks induction by GSE was also investigated by the comet assay and no significative difference was detected for treated and no treated DNA for 60 min. Our data suggest that GSE although no mutagenic presents cytotoxic activity.

摘要

通过不同生物测定法评估了从红葡萄美洲葡萄（伊莎贝尔品种）中提取的无酒精水醇性葡萄皮提取物（GSE）的毒性，该提取物具有抗高血压、血管舒张和抗氧化作用。使用鼠伤寒沙门氏菌/微粒体试验对TA97、TA98、TA100和TA102菌株进行检测，即使在有代谢作用的情况下，所有测试浓度（0.1 - 100微克/毫升）均未检测到致突变性。然而，在有和没有代谢作用的情况下，TA97和TA102以及在有代谢作用的情况下TA100均观察到细胞毒性。仅在存在代谢酶时，SOS - 显色试验中β - 半乳糖苷酶诱导的测量结果对于大肠杆菌PQ37才呈阳性。使用Balb/c 3T3成纤维细胞，通过彗星试验也研究了GSE对DNA链断裂的诱导作用，在处理60分钟后，处理过的DNA和未处理的DNA之间未检测到显著差异。我们的数据表明，GSE虽然没有致突变性，但具有细胞毒性活性。