汞膜电极上锇修饰DNA的伏安法：在检测DNA杂交中的应用

Voltammetry of osmium-modified DNA at a mercury film electrode: application in detecting DNA hybridization.

作者信息

Kostecka Pavel, Havran Ludek, Pivonkova Hana, Fojta Miroslav

机构信息

Laboratory of Biophysical Chemistry and Molecular Oncology, Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, Brno 612 65, Czech Republic.

出版信息

Bioelectrochemistry. 2004 Jun;63(1-2):245-8. doi: 10.1016/j.bioelechem.2003.11.005.

DOI:10.1016/j.bioelechem.2003.11.005

PMID:15110280

Abstract

Mercury film electrodes (MFE) have recently been used in nucleic acid electrochemical analysis as alternatives to the classical mercury drop ones. DNA modified with osmium tetroxide, 2,2'-bipyridine (Os,bipy) can be detected with a high sensitivity at mercury electrodes via measurements of a catalytic osmium signal. In this paper we show that mercury film on a glassy carbon electrode can be used in voltammetric analysis of Os,bipy-modified DNA. Application of the MFE as a detection electrode in double-surface electrochemical DNA hybridization assay involving osmium labeling of target DNA is demonstrated.

摘要

汞膜电极（MFE）最近已被用于核酸电化学分析，作为传统汞滴电极的替代品。用四氧化锇、2,2'-联吡啶（Os,bipy）修饰的DNA可通过测量催化锇信号在汞电极上以高灵敏度检测。在本文中，我们表明玻碳电极上的汞膜可用于Os,bipy修饰DNA的伏安分析。证明了MFE作为检测电极在涉及靶DNA锇标记的双面电化学DNA杂交测定中的应用。