类固醇21-羟化酶是参与成人自身免疫性艾迪生病的主要自身抗原。
Steroid 21-hydroxylase is a major autoantigen involved in adult onset autoimmune Addison's disease.
作者信息
Bednarek J, Furmaniak J, Wedlock N, Kiso Y, Baumann-Antczak A, Fowler S, Krishnan H, Craft J A, Rees Smith B
机构信息
Endocrine Immunology Unit, University of Wales College of Medicine, Heath Park, Cardiff, UK.
出版信息
FEBS Lett. 1992 Aug 31;309(1):51-5. doi: 10.1016/0014-5793(92)80737-2.
An adrenal-specific protein reacting with autoantibodies in the sera of patients with adult onset Addison's disease has been purified from human adrenal glands. The protein, mol.wt. 55K, has the biochemical characteristics of steroid 21-hydroxylase and reacts on Western blots with rabbit antibodies to recombinant 21-hydroxylase. Absorption of the native human 55K adrenal protein with human adrenal autoantibodies prevented the subsequent reaction of the 55K protein with rabbit antibodies to 21-hydroxylase in Western blot analysis. In addition, human adrenal autoantibodies reacted with recombinant 21-hydroxylase expressed in yeast. These data indicate that the adrenal specific enzyme steroid 21-hydroxylase is a major autoantigen involved in adult onset autoimmune Addison's disease.
已从人肾上腺中纯化出一种与成年期发病的艾迪生病患者血清中的自身抗体发生反应的肾上腺特异性蛋白。该蛋白分子量为55K，具有类固醇21-羟化酶的生化特性，在蛋白质印迹法中可与兔抗重组21-羟化酶抗体发生反应。在蛋白质印迹分析中，用人肾上腺自身抗体吸收天然的人55K肾上腺蛋白后，可阻止55K蛋白随后与兔抗21-羟化酶抗体发生反应。此外，人肾上腺自身抗体可与在酵母中表达的重组21-羟化酶发生反应。这些数据表明，肾上腺特异性酶类固醇21-羟化酶是成年期发病的自身免疫性艾迪生病中涉及的主要自身抗原。
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