Iwamoto Jun, Takeda Tsuyoshi, Ichimura Shoichi, Sato Yoshihiro, Yeh James K
Department of Sports Medicine, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
Yonsei Med J. 2004 Apr 30;45(2):314-24. doi: 10.3349/ymj.2004.45.2.314.
The purpose of this study was to clarify the differential effect of vitamin K and vitamin D supplementation on bone mass in young rats fed a normal or low calcium diet. Ninety female Sprague-Dawley rats, 6 weeks of age, were randomized by stratified weight method into nine groups with 10 rats in each group: baseline control, and 0.5% (normal) or 0.1% (low) calcium diet, either alone, or with vitamin K (30 mg/100g, food intake), vitamin D (25 micro g/100 g, food intake), or vitamin K + vitamin D. After 10 weeks of feeding, bone histomorphometric analyses were performed on cortical bone of the tibial shaft and cancellous bone of the proximal tibia. Vitamin K supplementation increased the maturation-related cancellous bone gain and retarded the reduction in the maturation-related cortical bone gain in rats fed a low calcium diet, and increased the maturation-related cortical bone gain in rats fed a normal calcium diet. Vitamin D supplementation reduced the maturation-related cancellous bone gain, prevented the reduction in periosteal bone gain, and enhanced the enlargement of the marrow cavity, with no significant effect on the reduction in the maturation-related cortical bone gain in rats fed a low calcium diet, and increased the maturation- related cancellous and cortical bone gains with increased periosteal bone gain in rats fed a normal calcium diet. An additive effect of vitamin K and vitamin D on the maturation- related cortical bone gain was found in rats fed a normal calcium diet. This study shows the differential effects of vitamin K and vitamin D supplementation on cancellous and cortical bone mass in young rats fed a normal or low calcium diet, as well as the additive effect on cortical bone under calcium sufficient condition.
本研究的目的是阐明补充维生素K和维生素D对正常钙饮食或低钙饮食的幼鼠骨量的差异影响。将90只6周龄的雌性斯普拉格-道利大鼠按分层体重法随机分为9组，每组10只：基线对照组，以及单独给予0.5%（正常）或0.1%（低）钙饮食，或同时给予维生素K（30mg/100g，食物摄入量）、维生素D（25μg/100g，食物摄入量），或维生素K + 维生素D。喂养10周后，对胫骨干皮质骨和胫骨近端松质骨进行骨组织形态计量学分析。补充维生素K可增加低钙饮食大鼠成熟相关的松质骨增加量，并延缓成熟相关的皮质骨增加量的减少，还可增加正常钙饮食大鼠成熟相关的皮质骨增加量。补充维生素D可减少成熟相关的松质骨增加量，防止骨膜骨增加量的减少，并促进骨髓腔扩大，对低钙饮食大鼠成熟相关的皮质骨增加量的减少无显著影响，可增加正常钙饮食大鼠成熟相关的松质骨和皮质骨增加量以及骨膜骨增加量。在正常钙饮食的大鼠中发现维生素K和维生素D对成熟相关的皮质骨增加有相加作用。本研究显示了补充维生素K和维生素D对正常钙饮食或低钙饮食的幼鼠松质骨和皮质骨量的差异影响，以及在钙充足条件下对皮质骨的相加作用。
