耐万古霉素肠球菌中VanA、VanB和VanC表型的快速检测与鉴别方法

Rapid detection and differentiation method of VanA, VanB and VanC phenotypes in vancomycin-resistant enterococci.

作者信息

Hanaki Hideaki, Yamaguchi Yoshio, Nomura Syuichi, Nagayama Ariaki, Sunakawa Keisuke

机构信息

Laboratory of Clinical Bacteriology, Department Nutrition Nakamuragakuen University, 5-7-1 Befu, Jyonan-ku, Fukuoka, Japan.

出版信息

Int J Antimicrob Agents. 2004 May;23(5):502-5. doi: 10.1016/j.ijantimicag.2003.09.031.

DOI:10.1016/j.ijantimicag.2003.09.031

PMID:15120731

Abstract

We developed a simple method that can replace the polymerase chain reaction (PCR) to distinguish between vancomycin-resistant enterococci (VRE) with the vanA, vanB and vanC genes. The method is based on induction of teicoplanin resistance by vancomycin in vanB-VRE, while the two compounds have a synergistic effect in vanC-VRE. In addition, vanA-VRE shows resistance to both vancomycin and teicoplanin, and both the compounds can induce resistance to vanA-VRE. Utilising these properties, we attempted to develop a simple method to distinguish between vanA, vanB and vanC. We compared our simple method with the PCR method in 43 strains of vanA-VRE, 35 strains of vanB-VRE and 37 strains of vanC-VRE. The results were 100% consistent with that obtained by PCR.

摘要

我们开发了一种简单的方法，该方法可以替代聚合酶链反应（PCR）来区分携带vanA、vanB和vanC基因的耐万古霉素肠球菌（VRE）。该方法基于万古霉素在vanB-VRE中诱导替考拉宁耐药性，而这两种化合物在vanC-VRE中具有协同作用。此外，vanA-VRE对万古霉素和替考拉宁均显示耐药性，且这两种化合物均可诱导vanA-VRE产生耐药性。利用这些特性，我们试图开发一种简单的方法来区分vanA、vanB和vanC。我们将我们的简单方法与PCR方法在43株vanA-VRE、35株vanB-VRE和37株vanC-VRE中进行了比较。结果与PCR方法所得结果100%一致。