他克林对培养的大鼠海马神经元延迟整流钾电流和瞬时外向钾电流的抑制作用
[Inhibition of tacrine on delayed rectifier and transient outward potassium currents in cultured rat hippocampal neurons].
作者信息
Zhang Wei, Jin Hong-wei, Xu Shao-feng, Qu Lin-tao, Wang Xiao-liang
机构信息
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
出版信息
Yao Xue Xue Bao. 2004 Feb;39(2):93-6.
AIM
To study the effects of tacrine on IK and IA potassium current in primary cultured rat hippocampal neurons.
METHODS
Whole cell patch clamp and primary rat hippocampal neuron cultures were used.
RESULTS
Tacrine was shown to reduce the amplitude of IK and IA, in concentration-dependent manners. The IC50s at +40 mV for reduction of IK and IA were 23 and 52.6 mumol.L-1, respectively. Tacrine (30 mumol.L-1) shifted the steady state activation of IK and IA to negative potentials by 12 and 15 mV, respectively. The V1/2 of activation curves for IK current before and after the application of tacrine were (6.7 +/- 1.4) mV and (-5.4 +/- 1.3) mV, respectively. The k of activation curves for IK current was 13.4 + 1.3 and 12.5 + 1.4 without and with tacrine, respectively. The V1/2 of activation curve for IA current were (-9.9 +/- 2.6) mV and (-24 +/- 5) mV in the absence and presence of tacrine, respectively, and the k value was not changed.
CONCLUSION
Tacrine inhibited IK and IA currents in rat hippocampal neurons and it is more potent for blocking IK.
目的
研究他克林对原代培养大鼠海马神经元IK和IA钾电流的影响。
方法
采用全细胞膜片钳技术和原代大鼠海马神经元培养方法。
结果
他克林呈浓度依赖性降低IK和IA的幅度。在+40 mV时，他克林降低IK和IA的半数抑制浓度（IC50）分别为23和52.6 μmol·L-1。他克林（30 μmol·L-1）分别使IK和IA的稳态激活电位向负电位移动12和15 mV。应用他克林前后IK电流激活曲线的半数激活电压（V1/2）分别为（6.7±1.4）mV和（-5.4±1.3）mV。IK电流激活曲线的斜率因子（k）在未用和应用他克林时分别为13.4 + 1.3和12.5 + 1.4。IA电流激活曲线的V1/2在未用和应用他克林时分别为（-9.9±2.6）mV和（-
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