戊糖乳杆菌自溶酶的特性分析
Characterisation of autolytic enzymes in Lactobacillus pentosus.
作者信息
Cibik R, Chapot-Chartier M-P
机构信息
Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, University of Uludag, Bursa, Turkey.
出版信息
Lett Appl Microbiol. 2004;38(6):459-63. doi: 10.1111/j.1472-765X.2004.01516.x.
AIMS
To characterize autolysis and autolytic system of the lactic acid bacterium Lactobacillus pentosus.
METHODS AND RESULTS
Autolysis of nine Lact. pentosus strains was evaluated in buffer solution. Their peptidoglycan hydrolase profiles were examined by renaturing SDS-PAGE and revealed two major activity bands at 58 and 112 kDa. Specificity analysis indicated the presence of at least two different types of peptidoglycan hydrolase activities in Lact. pentosus 1091.
CONCLUSIONS
Autolysis of Lact. pentosus was shown to be strain dependent and involvement of at least two different autolysins was evidenced.
SIGNIFICANCE AND IMPACT OF THE STUDY
The autolytic system of Lact. pentosus was characterized for the first time and the data obtained could be used in the selection of strains of technological interest.
目的
表征戊糖乳杆菌的自溶作用及自溶系统。
方法与结果
在缓冲溶液中评估了9株戊糖乳杆菌的自溶情况。通过复性SDS-PAGE检测其肽聚糖水解酶谱，结果显示在58 kDa和112 kDa处有两条主要活性带。特异性分析表明戊糖乳杆菌1091中存在至少两种不同类型的肽聚糖水解酶活性。
结论
戊糖乳杆菌的自溶作用表现出菌株依赖性，且证明至少有两种不同的自溶素参与其中。
研究的意义与影响
首次对戊糖乳杆菌的自溶系统进行了表征，所获得的数据可用于筛选具有技术应用价值的菌株。
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