Watanabe Katsuhiko, Nishida Kohji, Yamato Masayuki, Umemoto Terumasa, Sumide Taizo, Yamamoto Kazuaki, Maeda Naoyuki, Watanabe Hitoshi, Okano Teruo, Tano Yasuo
Department of Ophthalmology, Osaka University Medical School, Room E7, Yamadaoka 2-2, Suita, Osaka 565-0871, Japan.
FEBS Lett. 2004 May 7;565(1-3):6-10. doi: 10.1016/j.febslet.2004.03.064.
Many types of organ-specific stem cells have been recently shown to exhibit a side population (SP) phenotype based on their ability to efflux Hoechst 33342 dye. Because stem cells from corneal epithelium reside in the basal layer of the limbal epithelium, the purpose of this study was to examine whether the limbal epithelium contains SP cells. The ATP-binding cassette transporter Bcrp1/ABCG2 is reported to contribute to the SP phenotype in cells from several diverse sources. Here we show data from fluorescence-activated cell sorting and real-time quantitative RT-PCR analysis showing that harvested limbal epithelial cells contain SP cells expressing ABCG2. Immunofluorescence revealed that a portion of limbal epithelial basal cells expressed ABCG2. Data indicate that ABCG2 positive limbal epithelial cells are putative corneal epithelial stem cells.
最近研究表明，许多类型的器官特异性干细胞基于其排出Hoechst 33342染料的能力呈现出侧群（SP）表型。由于角膜上皮干细胞位于角膜缘上皮的基底层，本研究旨在检测角膜缘上皮是否含有SP细胞。据报道，ATP结合盒转运体Bcrp1/ABCG2在多种来源的细胞中促成SP表型。在此，我们展示了荧光激活细胞分选和实时定量RT-PCR分析的数据，结果显示收获的角膜缘上皮细胞含有表达ABCG2的SP细胞。免疫荧光显示，一部分角膜缘上皮基底细胞表达ABCG2。数据表明，ABCG2阳性的角膜缘上皮细胞是推测的角膜上皮干细胞。
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