热休克蛋白25（Hsp25）是热休克蛋白30家族的成员之一，在应激反应中促进包涵体的形成。
Hsp25, a member of the Hsp30 family, promotes inclusion formation in response to stress.
作者信息
Katoh Yumiko, Fujimoto Mitsuaki, Nakamura Kosuke, Inouye Sachiye, Sugahara Kazuma, Izu Hanae, Nakai Akira
机构信息
Department of Biochemistry and Molecular Biology, Yamaguchi University School of Medicine, Minami-Kogushi 1-1-1, Ube 775-8505, Japan.
出版信息
FEBS Lett. 2004 May 7;565(1-3):28-32. doi: 10.1016/j.febslet.2003.12.085.
Protein aggregates are oligomeric complexes of misfolded proteins, and serve as the seeds of inclusion bodies termed aggresomes in the cells. Heat shock proteins (Hsps) prevent misfolding and aggregate formation. Here, we found that only avian Hsp25 dominantly accumulated in the aggresomes induced by proteasome inhibition. Molecular cloning of chicken Hsp25 (cHsp25) revealed that it belongs to the Hsp30 family, which is a subfamily of the alpha-crystallin/small Hsp gene family. Unexpectedly, overexpression of cHsp25 into HeLa cells promoted inclusion formation whereas overexpression of mouse Hsp27 and its chicken homologue did not. These results suggest that cHsp25 acts differently from other small Hsps on protein aggregates.
蛋白质聚集体是错误折叠蛋白质的寡聚复合物，在细胞中作为被称为聚集体的包涵体的种子。热休克蛋白（Hsps）可防止错误折叠和聚集体形成。在此，我们发现只有禽类Hsp25在蛋白酶体抑制诱导的聚集体中大量积累。鸡Hsp25（cHsp25）的分子克隆表明它属于Hsp30家族，该家族是α-晶状体蛋白/小Hsp基因家族的一个亚家族。出乎意料的是，将cHsp25过表达到HeLa细胞中会促进包涵体形成，而小鼠Hsp27及其鸡同源物的过表达则不会。这些结果表明，cHsp25在蛋白质聚集体上的作用与其他小Hsps不同。
Zotero 插件