采用反相液相色谱法结合固相萃取法同时测定人血浆中艾司洛尔及其酸性代谢物的对映体。
Simultaneous determination of the enantiomers of esmolol and its acid metabolite in human plasma by reversed phase liquid chromatography with solid-phase extraction.
作者信息
Tang Yi-Hong, He Ying, Yao Tong-Wei, Zeng Su
机构信息
Department of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310031, PR China.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Jun 15;805(2):249-54. doi: 10.1016/j.jchromb.2004.03.007.
A stereoselective RP-high performance liquid chromatography (HPLC) assay to determine simultaneously the enantiomers of esmolol and its acid metabolite in human plasma was developed. The method involved a solid-phase extraction and a reversed-phase chromatographic separation with UV detection (lambda = 224 nm) after chiral derivatization. 2,3,4,6-tetra-O-acetyl-beta-d-glucopyranosyl isothiocyanate (GITC) was employed as a pre-column chiral derivatization reagent. The assay was linear from 0.09 to 8.0 microg/ml for each enantiomer of esmolol and 0.07-8.0 microg/ml for each enantiomer of the acid metabolite. The absolute recoveries for all enantiomers were >73%. The intra- and inter-day variations were <15%. The validated method was applied to quantify the enantiomers of esmolol and its metabolite in human plasma for hydrolysis studies.
建立了一种立体选择性反相高效液相色谱（HPLC）法，用于同时测定人血浆中艾司洛尔及其酸性代谢物的对映体。该方法包括固相萃取和手性衍生化后采用紫外检测（λ = 224 nm）的反相色谱分离。2,3,4,6-四-O-乙酰基-β-D-吡喃葡萄糖基异硫氰酸酯（GITC）用作柱前手性衍生化试剂。艾司洛尔各对映体的测定线性范围为0.09至8.0 μg/ml，酸性代谢物各对映体的测定线性范围为0.07至8.0 μg/ml。所有对映体的绝对回收率均>73%。日内和日间变化均<15%。该验证方法用于定量人血浆中艾司洛尔及其代谢物的对映体，以进行水解研究。
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